Part:BBa_K1470005

engineered PDZ domain (ePDZ)

Usage and Biology

The engineered PDZ domain is a globuar protein domain of 80 - 90 amino acids wich has an enhanced interaction with other proteins. Normally PDZ domains bind to a short region at the C-terminus of other proteins but there are some proteins which recognize internal sequence motifs of target proteins through a single binding site on the domains [1][2]. There are more over 1000 known proteins containing this domain in eucaryotes and bacteria but just a few examples are shown in archea [3].
ePDZ is a crucial part of the blue light-inducible expression system. It is able to bind to the J-alpha-helix of the LOV2 domain. The principle of the light-induced expression is explained here.

References

[1] Cowburn D (December 1997). „Peptide recognition by PTB and PDZ domains“. Curr. Opin. Struct. Biol. 7 (6): 835–838
[2] Giallourakis C, Cao Z, Green T, Wachtel H, Xie X, Lopez-Illasaca M, Daly M, Rioux J, Xavier R. A molecular-properties-based approach to understanding PDZ domain proteins and PDZ ligands. Genome Res. 2006;16:1056–1072.
[3] Doyle DA, Lee A, Lewis J, Kim E, Sheng M, MacKinnon R (June 1996). „Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ“. Cell 85 (7): 1067–1076
[4] MUELLER, K., ENGESSER, R., TIMMER, J., ZURBRIGGEN, M. D., Weber W.: Orthogonal Optogenetic Triple-Gene Control in Mammalian Cells. ACS Synth. Biol., Article ASAP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 424
  
• 1000
  COMPATIBLE WITH RFC[1000]