Part:BBa_K1499500:Experience

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Applications of BBa_K1499500

Figure 2. Flow cytometry data for construct.

To measure the time delay created by the quorum sensing construct we used GFP as a visual reporter and measured change in fluorescence with time after induction with IPTG. After using a fluorometer to obtain preliminary data on changes in GFP expression, we used flow cytometry to quantify this change. In the graphs above it is visible that, although GFP expression was leaky, fluorescence increased 10-fold after induction with IPTG. Thus, the quorum sensing construct can be used to create an 18 hour time delay that increases expression of the gene following the luxPR promoter by an entire order of magnitude.

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