Composite
NemR URep

Part:BBa_K1398004

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-12)
Revision as of 19:19, 2 November 2014 by EdwardMuir (Talk | contribs)

NemR Intergenic Reporter

A construct created to test the effectiveness of the NemR upstream intergenic region (BBa_1398005). The construct contains the entire NemR upstream region (BBa_1398005), which contains a promoter and RBS. It is followed by the fluorescent reporter iLOV (listed in the repository as BBa_K660004), a double STOP codon and a double terminator (B0015).

No fluorescence response was observed when TNT was introduced in solution with the plasmid carrying bacteria.

Flu-OF_compared_to_0ul_TNT_as_the_level_of_TNT_in_a_culture_changes..png

The full article detailing the characterisation of this part can be found here:

http://2014.igem.org/Team:Exeter/Detection


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None