Part:BBa_K1332010

mRNA circularization device (3� side)

This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. But the 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.

explanation of this part

Figure 1. mRNA circularization device (3'side)(not endless translation)

Part which surrounded by red closing line is mRNA circularization device (3’side)(not endless translation).(figure 1)
This part has a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.

How to use

Insert the protein coding sequence between this device and mRNA circularization device (5’ side). How to use this part is same as how to use 5' side device(BBa_K1332008) or 3' side device(endless translation)(BBa_K1332009) . Thus,read that.

Synthesis of long-chain proteins

Summary of the experiment

Confirm repeating translation by SDS-PAGE([http://2014.igem.org/Team:Gifu/Protocol#SDS protocol]).

Result

Figure 2. The result of SDS-PAGE

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]