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Part:BBa_K1332010

Designed by: Kenta Nomura   Group: iGEM14_Gifu   (2014-09-26)
Revision as of 04:51, 2 November 2014 by Ybanno (Talk | contribs)

mRNA circularization device (3 side)

This part consists of the 5' side of the intron(+exon fragment) from td gene of T4 phage(BBa_K1332004) and a double terminator(BBa_B0015). The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side)(BBa_K1332008) can be circularized. The 5' side of the intron(+exon fragment) from td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.

explanation of this part

Gifu 5' not remove2.png
Figure 1. mRNA circularization device (3'side)(not endless translation)

Part which surrounded by red closing line is mRNA circularization device (3’side)(not endless translation).(figure 1)


How to use

Insert the protein coding sequence between this device and mRNA circularization device (5’ side). How to use this part is same as how to use 5' side device(BBa_K1332008) or 3' side device(endless translation)(BBa_K1332009) . Thus,read that.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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