Protein_Domain

Part:BBa_K1363002

Designed by: Bo Dong   Group: iGEM14_USTC-China   (2014-10-01)
Revision as of 02:21, 2 November 2014 by Zhenzhang (Talk | contribs)

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HfiA the Inhibitor of synthesis of polysaccharose

Introduction

HfiA is an inhibitor in the holdfast's polysaccharide synthesis pathway. It can inhibit one of the Hfs protein family-HfsJ. So it can block up the synthesis of polysaccharide. HfiA, It is regulated downstream of lovK-lovR and the relationship is as following: [Fig 1]

Fig 1

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

These results support a model in which HfiA inhibits holdfast development via direct interaction with an enzyme required for holdfast bio-synthesis.

Fig 2

Get this part

To get PompC-HfiA, we first PCR the HfiA from genome of C.Crescentus. Then we standardize it, sequenced it and the result is exactly right.

PCR result: HfiA together with two another motion control proteins-DgrA and DgrB
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None