Protein_Domain

Part:BBa_K1363000

Designed by: Bo Dong   Group: iGEM14_USTC-China   (2014-09-29)
Revision as of 02:19, 2 November 2014 by Zhenzhang (Talk | contribs)

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DgrA the receptor of c-di-GMP

Introduction

The concentration of c-di-GMP is varying, and for our experiment we will promote its expression to inhibit rotation of flagella. It will bind to DgrA and DgrB. The resulting complex will have two routes to go: one is the [c-di-GMP& DgrA] complex which will inhibit FliL, a protein bounds to the membrane playing a key role in rotation of flagella. The other route is that the DgrB will directly affect the rotation of the flagella.

Fig 1: A dimer of ci-d-GMP binds to the surface of a homolog of DgrA

Get the part

Fig 2

We get this part by PCR its gene from the genome of C.Crescentus. The standard parts construction have been accomplished, which helps motion regulation and holdfast biosynthesis of bacteria.

Reference

DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 199
    Illegal NgoMIV site found at 348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


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Categories
Parameters
n/aDgrA the receptor of c-di-GMP