Part:BBa_K1391016

pENTR_hEF1a

Strong constitutive promoter for mammalian gene transcription. This part is a repeat of BBa_K779200 .

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 1211
Illegal PstI site found at 338
Illegal PstI site found at 843
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 1211
Illegal PstI site found at 338
Illegal PstI site found at 843
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 1211
Illegal BglII site found at 592
Illegal BamHI site found at 1198
Illegal XhoI site found at 991
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 1211
Illegal PstI site found at 338
Illegal PstI site found at 843
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 7
Illegal EcoRI site found at 1211
Illegal PstI site found at 338
Illegal PstI site found at 843
Illegal NgoMIV site found at 726
Illegal AgeI site found at 104
1000
COMPATIBLE WITH RFC[1000]

hEF1a in the context of MIT iGEM 2014

MIT iGEM 2014 used hEF1a to express many constructs constitutively in HEK293. One way in which we used the hEF1a promoter was to express constitutive colors as transfection markers, to give us an indication of how many plasmids were transfected into our cells. Here we transfected our cells with hEF1a:mKate and hEF1a:tagBFP, red and blue fluorescent proteins. As you can see, there is a linear correlation between the amount of mKate and tagBFP expression that we observe.

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