Coding

Part:BBa_K1539002:Experience

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-09)
Revision as of 20:53, 1 November 2014 by Stassoulas3 (Talk | contribs) (→‎Applications of BBa_K1539002)


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Applications of BBa_K1539002

BBa_K1539001 was created using devices BBa_K1539034 and BBa_K1539089 to insert relatively low expressing promoters and low efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry, and the median intensity was 4X dimmer than a high promoter/high RBS combination (see part [1]). Our raw data is summarized below:

"Low Expression Combination" - Primers PL1/RL1:

File:140821 DMC mCherry PL1-RL1.pdf

Negative Control:

File:140821 DMC mCherry Promoterless.pdf

User Reviews

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