Project
EWOBS

Part:BBa_J45800

Designed by: MIT iGEM 2006   Group: iGEM2006_MIT   (2006-10-31)
Revision as of 06:36, 31 October 2006 by Jasonk (Talk | contribs)

Exponential phase wintergreen odor biosynthetic system

This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent). It uses a stationary phase promoter followed by an inverter (see BBa_J45996 for details) to only express the necessary enzymes during exponential phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3161


[edit]
Categories
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Parameters
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