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Part:BBa_K1413041:Design

Designed by: Sophia Belkhelfa   Group: iGEM14_Evry   (2014-10-07)
Revision as of 18:13, 1 November 2014 by Chesne j (Talk | contribs) (→‎References)

Mutation of OriVR6Kgamma origin of replication (ori)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part follows rules of RFC92, compatible with RFC10. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the Ori sequence. Hence, the oriVR6Kgamma can only be replicated in a bacterial strain producing the pi protein. This ori was already used in the iGEM competition in 2009 by a french team. The gamma origin is adjacent to the pi protein binding site and other sites bounded by the host cell proteins involved in its own reproduction.


Source

This part was given by a member of the institute of systems and synthetic biology (Evry, France)

References

Matsumoto-Mashimo C1, Guerout AM, Mazel D. (2004) A new family of conditional replicating plasmids and their cognate Escherichia coli host strains, Research in Microbiology