Part:BBa_J45200

Banana odor generator

Protein generator producing the protein ATF1 under control of the LacI repressor. Thus, in a LacI^- E. coli strain, ATF1 is continually produced. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol). This part is almost identical to BBa_J45220 except that it uses the B0030 RBS (instead of B0032 as is in BBa_J45220). The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 182
  Illegal BamHI site found at 1509
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 609
  Illegal SapI.rc site found at 1617