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Regulatory

Part:BBa_K1351038

Designed by: Nikolai Peschek   Group: iGEM14_LMU-Munich   (2014-10-06)
Revision as of 04:55, 1 November 2014 by JudithHeckmann (Talk | contribs)

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P3-promoter

Quorum sensing dependant promotor (AIP II). Regulated by phosphorylated AgrA. Contains four AgrA binding sites upstream. This part belongs to the of the AIP-II sensing two component system AgrCA. Upon detection of autoinducing peptide II (AIP-II), AgrC phosphorylates AgrA, which then bindes to distinct binding sites and activates the P2 and P3 [this part] promoters.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

LMU14 Cap2p3.png

Luminescence of B. subtilis PxylA - agrCA / P2-lux and P3-lux was measured in MCSE induced with 0, 0.02, 0.2 % xylose. This plot shows the luminescence readout normalised over OD600 over the course of 6hours. The higher output in xylose induced samples shows the successfull expression of agrCA in B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//regulation
Parameters
None