Device

Part:BBa_K812012:Experience

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)
Revision as of 02:33, 31 October 2014 by Vzepeda (Talk | contribs) (→‎Applications of BBa_K812012)


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Applications of BBa_K812012

The Nevada 2014 iGEM team used TIR1 in their yeast protein degradation project. We cloned TIR1 behind a constitutive yeast promoter (pADH1). We added a 9xMyc tag to help verify expression. After integrating our construct into W303 yeast cells, we were able to verify expression via western blot.

The results can be seen on our team page: [http://2014.igem.org/Template:Nevada/TheAuxinSystem TIR1 Expression by Western Blot]

File:Https://static.igem.org/mediawiki/2014/2/27/Team nevada research western blot for coi1.png

User Reviews

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