Project
SBOBS

Part:BBa_J45600:Design

Designed by: MIT iGEM 2006   Group: iGEM2006_MIT   (2006-10-30)
Revision as of 04:48, 31 October 2006 by Jasonk (Talk | contribs) (→‎Design Notes)


Stationary phase banana odor biosynthetic system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3204
    Illegal EcoRI site found at 4105
    Illegal EcoRI site found at 4804
    Illegal EcoRI site found at 4990
    Illegal SpeI site found at 3898
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3204
    Illegal EcoRI site found at 4105
    Illegal EcoRI site found at 4804
    Illegal EcoRI site found at 4990
    Illegal SpeI site found at 3898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3204
    Illegal EcoRI site found at 4105
    Illegal EcoRI site found at 4804
    Illegal EcoRI site found at 4990
    Illegal BglII site found at 327
    Illegal BamHI site found at 1654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3204
    Illegal EcoRI site found at 4105
    Illegal EcoRI site found at 4804
    Illegal EcoRI site found at 4990
    Illegal SpeI site found at 3898
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3204
    Illegal EcoRI site found at 4105
    Illegal EcoRI site found at 4804
    Illegal EcoRI site found at 4990
    Illegal SpeI site found at 3898
    Illegal AgeI site found at 3068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 754
    Illegal SapI.rc site found at 1762


Design Notes

We recommend use of strain BBa_J45999 as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the banana scent produced by J45600.

Source

This system is composed of devices J45250 and J45400.

References