Generator
Banana

Part:BBa_J45200:Design

Designed by: André Green II   Group: iGEM06_MIT   (2006-10-20)
Revision as of 04:34, 31 October 2006 by Drephd (Talk | contribs)

Banana odor generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617


Design Notes

We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.

Source

References