Plasmid

Part:BBa_K1391131:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 16:19, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)


pEXPR_TRE:miRNAG2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5166
    Illegal EcoRI site found at 5786
    Illegal EcoRI site found at 5827
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 6364
    Illegal PstI site found at 7013
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5166
    Illegal EcoRI site found at 5786
    Illegal EcoRI site found at 5827
    Illegal NheI site found at 3210
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 6364
    Illegal PstI site found at 7013
    Illegal NotI site found at 6669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5166
    Illegal EcoRI site found at 5786
    Illegal EcoRI site found at 5827
    Illegal BglII site found at 5907
    Illegal BamHI site found at 2854
    Illegal BamHI site found at 3328
    Illegal BamHI site found at 4632
    Illegal BamHI site found at 6864
    Illegal XhoI site found at 3538
    Illegal XhoI site found at 5062
    Illegal XhoI site found at 6649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5166
    Illegal EcoRI site found at 5786
    Illegal EcoRI site found at 5827
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 6364
    Illegal PstI site found at 7013
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3580
    Illegal EcoRI site found at 4233
    Illegal EcoRI site found at 4598
    Illegal EcoRI site found at 5166
    Illegal EcoRI site found at 5786
    Illegal EcoRI site found at 5827
    Illegal XbaI site found at 4525
    Illegal PstI site found at 2677
    Illegal PstI site found at 3656
    Illegal PstI site found at 3939
    Illegal PstI site found at 6364
    Illegal PstI site found at 7013
    Illegal NgoMIV site found at 2297
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4024
    Illegal AgeI site found at 3456
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1121
    Illegal BsaI.rc site found at 5688
    Illegal SapI site found at 38
    Illegal SapI site found at 3214
    Illegal SapI site found at 7096
    Illegal SapI.rc site found at 4680


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Artificial

References