Plasmid

Part:BBa_K1391101:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 16:14, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)

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pEXPR_Gantenerumab Variable Human Ig-M Light Chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 532
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 532
    Illegal AgeI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gantenerumab has shown therapeutic potential in recent clinical trials. Human Ig-M can be anchored to the membrane to interact with the b-cell receptor (BCR) complex. Human antibody membrane localization tag is natively found in human, membrane-bound antibodies.

This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts.

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

(Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)

Source

Fusion protein of 3 human peptide sequences


References