Plasmid

Part:BBa_K1391029:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 15:55, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)

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pENTR_BACE2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 991
    Illegal EcoRI site found at 1038
    Illegal PstI site found at 250
    Illegal PstI site found at 873
    Illegal PstI site found at 1316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 991
    Illegal EcoRI site found at 1038
    Illegal PstI site found at 250
    Illegal PstI site found at 873
    Illegal PstI site found at 1316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 991
    Illegal EcoRI site found at 1038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 991
    Illegal EcoRI site found at 1038
    Illegal PstI site found at 250
    Illegal PstI site found at 873
    Illegal PstI site found at 1316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 991
    Illegal EcoRI site found at 1038
    Illegal PstI site found at 250
    Illegal PstI site found at 873
    Illegal PstI site found at 1316
    Illegal NgoMIV site found at 703
    Illegal NgoMIV site found at 1159
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 634


Design Notes

This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human

References