Plasmid

Part:BBa_K1391028:Design

Designed by: Shinjini Saha   Group: iGEM14_MIT   (2014-10-17)
Revision as of 15:55, 30 October 2014 by Clrichar (Talk | contribs) (→‎Design Notes)

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pENTR_BACE1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 637
    Illegal PstI site found at 911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 637
    Illegal PstI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 637
    Illegal PstI site found at 911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 637
    Illegal PstI site found at 911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 136
    Illegal SapI.rc site found at 145


Design Notes

This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human

References