Measurement

Part:BBa_K1510820

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-07)
Revision as of 20:58, 28 October 2014 by Sunnyyuyu (Talk | contribs)

RFP indicated INPNC performance evaluation

We anneal a RFP at the end of our INPNC display gene.We adopted a new method that measuring fluorescence light and O.D value of our modified e-coli to test the efficiency of INPNC expression. We successfully derived INPNC expression quantity and expression curve though time. And successfully proved that our targeted gene CSP16 is on the surface of our modified e-coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 2220
    Illegal AgeI site found at 2332
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None