Part:BBa_K1403015

Trimethylamine moonoxygenase (tmm) expression cassette This Biobrick encodes trimethylamine mono-oxygenase (tmm) which transforms trimethylamine into trimethylamin-N-oxide. This enzyme is originally expressed by Ruegeria pomeroyi and is similar to human FMO3.

Trimethylamine + NADPH + H+ + O2 = Trimethylamine-N-oxide + NADP+ + H2O

• Constitutive promoter BBa_J23108
• Synthetic RBS
• Histidine tag

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 438
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 262
  Illegal AgeI site found at 485
  
• 1000
  COMPATIBLE WITH RFC[1000]