Part:BBa_K1333317

I13458-I13453-FourU-RFP [[File:]]

Usage and Biology

Considering of the different transcription level provided by B2H system, we replace the constitutive promoter(BBa_J23119) in BBa_K1333309 by an inducible promoter, for using different concentration of inducer to simulate this situation. Avoiding destructing the structure of RNAT, we choose the pBAD(BBa_K1333315),induced by L-arabinose, whose operon are in the upstream of promoter.

In the 2014 distribution of biobricks, we found the parts of the promoter: arabinose C (AraC) with Promoter C (PC) (BBa_I13458) and PBad (BBa_I13453) modified by MIT in 2005, and integrated the two parts into araBAD operator (BBa_K1333315). And then, we constructed this araBAD operator before FourU element and RFP. [[File:IIFR-1-2.jpg]400px|thumb|left|Figure1. The inducible mRFP expression with RNAT-fourU element under PBad.]] Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1247
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1187
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 1873
  Illegal AgeI site found at 1985
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961