Part:BBa_K1423006
Arsenic Inducible Banana Odor Generator
The arsenic inducible banana odor generator consists of the arsenic inducible promoter, J33201, and the composite part J45199, which is made up of a ribosome binding site, the ATF1 gene, and a double terminator. E. coli transformed with this part can convert isoamyl alcohol to isoamyl acetate in the presence of heavy metals such as arsenic and cadmium. The generator may express false results, as the generator will produce isoamyl acetate without the presence of arsenic or other heavy metals. But expression appears to rise until a concentration of 5uM arsenite is injected. Any concentrations larger than this appear to be toxic to E.Coli cells, as the production of isoamyl alcohol drops significantly.
![[1]](https://static.igem.org/mediawiki/2014/b/b6/GCMSresults%282%29.png){kind=link}
The link above leads to a quantification of the production of isoamyl acetate normalized to the same construct with the high strength constitutive promoter J23100.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
Illegal BglII site found at 646
Illegal BamHI site found at 1973 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1073
Illegal SapI.rc site found at 2081
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