Coding

Part:BBa_K1351017

Designed by: Philipp Popp & Roman Herzog   Group: iGEM14_LMU-Munich   (2014-09-09)
Revision as of 09:24, 27 October 2014 by Phil234 (Talk | contribs)

SdpI with RBS: Immunity against the cannibalsim toxin sdpC of B. subtilis

This gene codes for the immunity against a cannibalism toxin SdpC of Bacillus subtilis. The sdpI gene is regulated by a separate promoter (PsdpIR) which is responsible for the operon sdpIR. Cannibalsim toxins are produced by B. subtilis mainly in mid to late stationary phase when nutrients are limited. The LMU-iGEM team also created an antisense mRNA BBa_K1351019 for the Bakillus module "suicide".

Prefix: TGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].


First results of this BioBrick are depicted in the following graph:

LMU14Sdpi.PNG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
Parameters
n/aSdpI with RBS: Immunity against the cannibalsim toxin sdpC of B. subtilis