Part:BBa_K1333108
DNAQ926 CDS
DNAQ926 coding sequence,lead to polymeraseIII proofreading defect in E.coli.
Usage and Biology
DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]
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