Generator

Part:BBa_K1499004

Designed by: Raman Nelakanti   Group: iGEM14_StanfordBrownSpelman   (2014-10-08)
Revision as of 20:01, 25 October 2014 by Rdispenza (Talk | contribs) (→‎Verification of Part)

Cellulose binding domains with streptavidin domain generator

This part encodes a protein with two cellulose-binding domains on either end joined by a streptavidin domain.


Usage and Biology

The idea behind this part is that each domain binds to a single cellulose fiber, thus providing a way to cross-link and strengthen cellulose polymers. In addition, the streptavidin domain allows for the modular addition of biotinylated proteins through protein-protein interactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 703
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 304
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 651

Characterization

Verification of Part

The part was sequence verified before submission to the registry with two reads using VF2 and VR (Figure 1).


[edit]
Categories
//binding/cellulose
Parameters
None