Coding

Part:BBa_K1371014:Design

Designed by: Qifeng Tan   Group: iGEM14_SCUT-China   (2014-10-07)
Revision as of 05:21, 25 October 2014 by Invly leung (Talk | contribs) (→‎References)

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accA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 808
    Illegal AgeI site found at 1165
    Illegal AgeI site found at 1297
    Illegal AgeI site found at 1492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 625
    Illegal BsaI.rc site found at 1339
    Illegal SapI.rc site found at 952


Design Notes

Source

Streptomyces coelicolor

References

[1]Rodrıguez E, Gramajo H. Genetic and biochemical characterization of the α and β components of a propionyl-CoA carboxylase complex of Streptomyces coelicolor A3 (2)[J]. Microbiology, 1999, 145(11): 3109-3119.

[2]Pfeifer B A, Admiraal S J, Gramajo H, et al. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli [J]. Science, 2001, 291(5509): 1790-1792.

[3]Oliynyk M, Samborskyy M, Lester J B, et al. Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338 [J]. Nature biotechnology, 2007, 25(4): 447-453.