Coding

Part:BBa_K1321103:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:15, 24 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)

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Phytochelatin (PC) EC20 + linker-CBDcipA-linker with T7 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 335
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

The promotor was cloned onto the protein via the XbaI/SpeI sites.

Source

Phytochelatin and CBDcipA was synthesized from Geneart and cloned into the psB1C3 backbone. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References