Coding

Part:BBa_K1321344:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 18:45, 24 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


NiBP fused to CBDcex in RFC 25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


Design Notes

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Source

The fusion protein was cloned together using the NgoMIV and AgeI sites. The promotor was cloned onto the protein via the XbaI/SpeI sites.

References