Illegal NgoMIV site found at 558 Illegal AgeI site found at 369
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1483
Design Notes
Source
Saccharopolyspora erythraea
References
[1]Cane, David E. Programming of erythromycin biosynthesis by a modular polyketide synthase [J]. Journal of Biological Chemistry, 2010, 285.36: 27517-27523.
[2]Komaki, Hisayuki, et al. Genome based analysis of type-I polyketide synthase and nonribosomal peptide synthetase gene clusters in seven strains of five representative Nocardia species [J]. BMC genomics 15.1 (2014): 323.
[3]Pfeifer, Blaine A., et al. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli [J]. Science, 2001, 291.5509: 1790-1792.
[4]Tae, Hongseok, Jae Kyung Sohng, and Kiejung Park. Development of an analysis program of type I polyketide synthase gene clusters using homology search and profile hidden Markov model [J]. Journal of microbiology and biotechnology, 2009, 19.2: 140-146.
[5]Cortes, Jesus, et al. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea [J]. 1990: 176-178.
[6]Khosla, Chaitan, Shiven Kapur, and David E. Cane. Revisiting the modularity of modular polyketide synthases [J]. Current opinion in chemical biology, 2009, 13.2: 135-143.
[7]Menzella, Hugo G., et al. Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster [J]. Journal of Industrial Microbiology and Biotechnology,2006, 33.1: 22-28.
[8]Oliynyk, Markiyan, et al. A hybrid modular polyketide synthase obtained by domain swapping [J]. Chemistry & biology,1996, 3.10: 833-839.
[9]Lau, Janice, David E. Cane, and Chaitan Khosla. Substrate specificity of the loading didomain of the erythromycin polyketide synthase [J]. Biochemistry, 2000, 39.34: 10514-10520.
[10]Nowak-Thompson, Brian, et al. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5 [J]. Journal of bacteriology, 1999, 181.7: 2166-2174.
[11]Caffrey, Patrick, et al. Amphotericin biosynthesis in Streptomyces nodosus deductions from analysis of polyketide synthase and late genes [J]. Chemistry & biology,2001, 8.7: 713-723. [12]Dunn, Briana J., et al. Comparative analysis of the substrate specificity of trans-versus cis-acyltransferases of assembly line polyketide synthases [J]. Biochemistry,2014.
[13]Jiang, Ming, and Blaine A. Pfeifer. Metabolic and pathway engineering to influence native and altered erythromycin production through E. Coli [J]. Metabolic engineering, 2013, 19: 42-49. [14]Chen, Xianzhong, et al. Metabolic engineering of Escherichia coli: A sustainable industrial platform for bio-based chemical production [J]. Biotechnology advances, 2013, 31.8: 1200-1223.
