Composite
Part:BBa_J36847
Designed by: Perry Tsai Group: iGEM06_Harvard (2006-10-30)
Lpp-OmpA(46-159)-Streptavidin single-chain dimeric + His6
Assembled construct of signal peptide of lipoprotein, five transmembranes domain of outer membrane protein A, and streptavidin, single-chain dimeric with His6 tag at the end.
This construct codes for a protein which displays streptavidin on the cell surface.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1232
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1241
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 662
Illegal AgeI site found at 1196 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |