Part:BBa_K1493804
promoter with CIλ and Tet operator sites
Promoter designed to have operators sites for the tetracycline repressor and the cI lambda repressor, based on sequences in Elowitz et. al. 2007
This promoter contains two Tet operator sites at the distal and proximal position (left and right part of the promoter) and one cI lambda operator sites at the core site (middle part of the promoter).
Usage and Biology
The promoter was combined with GFP to check for its functionality.
Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.
Figure 1 shows the P4 (promoter with CIλ and Tet operator sites) expressing GFP, suggesting that it is a functional promoter.
This Promoter is part of a promoter set used in [http://2014.igem.org/Team:Wageningen_UR BananaGuard] listed here:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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