Part:BBa_K1493803
promoter with CIλ and LacI operator sites
Promoter designed to have operators sites for the Lac repressor and the cI lambda repressor, based on sequences in Elowitz et. al. 2007
This promoter contains two LacI operator sites at the core and proximal position (middle and right part of the promoter) and two cI lambda operator sites at the distal site (left part of the promoter).
Usage and Biology
The promoter was combined with GFP to check for its functionality.
Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.
Figure 1 shows the P3 (promoter with CIλ and LacI operator sites), it is not expressing GFP suggesting that it is either non functional or a very weak promoter.
This Promoter is part of a promoter set used in [http://2014.igem.org/Team:Wageningen_UR BananaGuard] listed here:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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