Part:BBa_K1493802

Promoter with Tet operator site

Promoter designed to have operators sites for the tetracycline repressor, based on sequences in Elowitz et. al. 2007

This promoter contains two TetR operator sites at the distal and proximal position (left and right part of the promoter)

Usage and Biology

The promoter was combined with GFP to check for its functionality. Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.

Figure 1 shows the P2 (Promoter with Tet operator site) expressing GFP, suggesting that it is a functional promoter.

This Promoter is part of a promoter set used in [http://2014.igem.org/Team:Wageningen_UR BananaGuard] listed here:

BBa_K1493801

BBa_K1493803

BBa_K1493804

BBa_K1493805

BBa_K1493806

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]