Coding

Part:BBa_K1499000

Designed by: Jotthe Kannappan   Group: iGEM14_StanfordBrownSpelman   (2014-08-27)
Revision as of 02:56, 24 October 2014 by Bdoughty (Talk | contribs)

wssF gene from wss operon

wssF is one of the four genes isolated from Pseudomonas fluorescens responsible for acetylating cellulose (the others being wssG-I, K149001-3). The wss operon contains 10 genes responsible for biofilm production in P. fluorescens, which allows the bacterium to colonize the air-liquid interface. Many of these genes have homologies in E. coli or G. hansenii, including minD, subunits of cellulose synthase, and a cellulase. Only four of the genes (wssF-I), however, are responsible for the acetylation of the cellulose polymer.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None