Composite
Part:BBa_M36921:Design
Designed by: Leah Chase Group: Stanford BIOE44 - S11 (2014-10-22)
Zinc Sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 194
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 194 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 190
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 194
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 194
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to enter silent mutations to change the DNA sequence of our gene without affecting the amino acid sequence because there were palindromes greater than 10 bp.
Source
The genomic sequences come from various parts of an E. Coli protein (ZntR).