Translational_Unit

Part:BBa_K1321335:Experience

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 07:18, 23 October 2014 by Ld1411 (Talk | contribs) (→‎Applications of BBa_K1321335)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1321335

Part BBa_K1321335 was digested with XbaI and PstI and the resulting fragment, containing the optimised coding sequences of AcsC and AcsD, was cloned into a medium-to-low copy number plasmid (pSB3K3) containing the inducible pLAC promoter. The resulting construct, verified by GreenTaq Colony PCR, was subsequently sub-cloned into BBa_K1321336-containing electrocompetent cells. For more information on the characterisation procedure, click here: https://parts.igem.org/Part:BBa_K1321334:Experience

User Reviews

UNIQf43e2440d5c1093d-partinfo-00000000-QINU UNIQf43e2440d5c1093d-partinfo-00000001-QINU