Measurement

Part:BBa_J37016

Designed by: Tom Hinson   Group: iGEM06_Imperial   (2006-07-17)
Revision as of 15:01, 30 October 2006 by Cys (Talk | contribs)

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Predator Molecule Generator Sensing Test (GFP output)

Based around Imperial iGEM's Predator Cell in order to assay transfer function of pLux

  • [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct Full Report on our project site]

Usage:

  • This part was created by iGEM@Imperial 2006 to be used as the predator sensing test construct in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to characterize the sensitivity of the predator cell towards the prey moleculte Acyl-Homoserine Lactone (a quorum sensing molecule).

Biology:

  • In the presence of AHL, AHL and LuxR form a heterodimer, which activates the promoter luxpR and thus expression of the genes downstream of the promoter. Depending on the levels of AHL, the level of expression of LuxR and GFP vary.
  • The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Modelling transfer curve].
  • The part was successfully tested using this [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols/T9002 testing protocol] and [http://openwetware.org/wiki/IGEM:Imperial/Results/J37016 experimental data] from testing was documented.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1532


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