RNA

Part:BBa_K1555007:Design

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-10-15)
Revision as of 06:40, 22 October 2014 by Xuan (Talk | contribs) (→‎Source)

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copA's 20nt target sequence+sgRNA scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used overlap PCR to link 20nt target sequence with sgRNA scaffold.

Source

20nt target sequence was synthesized artificially

the scaffold was acquired by PCR from a plasimid made by CAS

References