Regulatory

Part:BBa_K747096:Experience

Designed by: Lucas Schneider   Group: iGEM12_Freiburg   (2012-09-26)
Revision as of 01:29, 22 October 2014 by Gabyqz (Talk | contribs) (→‎CMV promoter fused with YFP by BIOSINT_Mexico iGEM 2014)


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Applications of BBa_K747096

Characterization by BIOSINT_Mexico iGEM 2014

CMV promoter fused with YFP by BIOSINT_Mexico iGEM 2014

As a part of the project from 2014_BIOSINT_Mexico , we fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030).

In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1]

Figure 1 YFP intensity every 45 minutes.














We made three repetitions, so in order to model the equation, we obtained the mean measure, therefore:


Figure 2 Media YFP intensity every 45 minutes.






We analysed the data and using the Wolfram Mathematica software, we obtained the following equation that fits the data. If we plot this equation, we obtain:

Model1res.png

Where we can see that the intensity of YFP grows directly proportional to the time and concentration of the molecule.

Figure 3 Intensity YFP vs time.


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