Signalling

Part:BBa_J37015

Designed by: Tom Hinson   Group: iGEM06_Imperial   (2006-07-17)
Revision as of 14:23, 30 October 2006 by Farah (Talk | contribs) (→‎Usage and Biology)

Prey Molecule Generator (AHL) + GFP Reporter

Prey Genotype + Reporter

Usage and Biology

Usage:

  • This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule) using a positive feedback loop.

Biology:

  • The positive feedback loop relies on leaky expression of LuxR by the tetR promoter which causes production of luxR. AHL already present in the cell forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI enzymatically produces AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
  • The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2400


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Parameters
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