Coding

Part:BBa_K1321359:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 23:37, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)

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sfGFP fused to dCBD driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 245


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

The LacI part came from BBa_J04500.

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References