Coding

Part:BBa_K1321359:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 23:37, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


sfGFP fused to dCBD driven by LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 245


Design Notes

sadf


Source

The LacI part came from BBa_J04500.

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References