Coding

Part:BBa_K1321341:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 23:18, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)


sfGFP fused to CBDclos in RFC 25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

asdfad

References