Coding

Part:BBa_K1321150:Design

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 22:00, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)

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Metallothionein Fmt fused to linker-dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 204


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format. dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References