Coding

Part:BBa_K1321150:Design

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 22:00, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)


Metallothionein Fmt fused to linker-dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 204


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

GATC

References