Coding

Part:BBa_K1321114:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:37, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Design Notes)


CBDclos fused to phytochelatin (PC) EC20 with LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CBD4+PhytoC with LacI

Source

Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. The LacI part came from BBa_J04500.

References