Coding

Part:BBa_K1321110:Design

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-09)
Revision as of 20:14, 21 October 2014 by Gabi1234 (Talk | contribs) (→‎Source)


Phytochelatin (PC) EC20 fused to linker-dCBD with LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

The LacI part was made by inverse PCR of BBa_J04500, and ligated.

References