Composite

Part:BBa_K1321336

Designed by: Laura de Arroyo Garcia   Group: iGEM14_Imperial   (2014-10-08)
Revision as of 16:59, 21 October 2014 by Ld1411 (Talk | contribs)

AraC-pBAD-AcsAB

This composite part is the result of combining parts BBa_K1321333 and BBa_K1321334 by BioBrick cloning. It contains the Arabinose-inducible promoter pBAD and also codes for the transcriptional inhibitor/activator AraC. Furthermore, pBAD is followed by the AcsAB open reading frame, which in turn codes for the cellulose synthase enzyme, key player for cellulose production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3049
    Illegal BglII site found at 3763
    Illegal BglII site found at 5686
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2059
    Illegal BamHI site found at 5053
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3035
    Illegal AgeI site found at 3112
    Illegal AgeI site found at 3668
    Illegal AgeI site found at 4469
    Illegal AgeI site found at 4561
    Illegal AgeI site found at 4667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None